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igd af488  (SouthernBiotech)


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    Structured Review

    SouthernBiotech igd af488
    Igd Af488, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 93/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/igd+alexa+fluor+488/Goat+Anti-Human+IgD-Alexa+Fluor+488/pmc13000230-395-76-79
    Average 93 stars, based on 17 article reviews
    igd af488 - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Staining:

    Article Title: Self-assembling influenza nanoparticle vaccines drive extended germinal center activity and memory B cell maturation
    Article Snippet: Flow data were analyzed in FlowJo v10, and statistical assessment was performed in GraphPad Prism. .. To identify antigen-specific B cells and activated T FH cells, defrosted LN cell suspensions were stained with PR8 HA-PE and PR8 HA-BV421 probes along with the following surface panel: CD14 BV510 (M5E2; BioLegend), CD16 BV510 (3G8; BioLegend), streptavidin BV510 (BD), Live/Dead Aqua (Thermo Fisher Scientific), CD4 BV605 (L200; BD), IgG BV786 (G18-145; BD), CXCR5 PE-Cy7 (MU5UBEE; Invitrogen, Thermo Fisher Scientific), CD20–Alexa Fluor 700 (2H7; BD), PD-1 APC Fire (EH12-2H7; BioLegend), IgD–Alexa Fluor 488 (polyclonal; Southern Biotech), IgM BUV395 (G20-127; BD), and CD3 BUV737 (SP34-2; BD). .. For Bcl6–Alexa Fluor 647 (IG191E/A8; BioLegend) and Ki-67 BV650 (B56; BD) staining, cells were fixed, permeabilized, and stained using the BD Transcription Factor Buffer Kit according to the manufacturer’s instructions.

    Article Title: Coformulation with Tattoo Ink for Immunological Assessment of Vaccine Immunogenicity in the Draining Lymph Node.
    Article Snippet: Samples were acquired on a BD LSRFortessa using BD FACSDiva. .. For NHP B cell analysis, thawed LN single-cell suspensions were stained with LIVE/DEAD Aqua Viability Dye (Life Technologies) and the following surface Abs: IgD Alexa Fluor 488 (polyclonal, SouthernBiotech), CD20 allophycocyanin-Cy7 (2H7, BioLegend), CD14 BV510 (M5E2, BioLegend), CD3 Alexa Fluor 700 (SP34-2, BD), CD8a BV510 (RPA-T8, BioLegend), CD16 BV510 (3G8, BioLegend), CD10 BV510 (HI10a, BioLegend), IgG BV786 (G18-145, BD), CD95 BUV737 (DX2, BD), CD4 BV605 (L200, BD), CXCR5 PE-Cy7 (MU5UBEE, eBioscience), and PD-1 BV421 (EH12.2H7, BioLegend). .. S-specific B cells were identified using biotinylated SARS-CoV-2 S probe conjugated to streptavidin PE (Thermo Fisher Scientific).

    Cell Analysis:

    Article Title: Coformulation with Tattoo Ink for Immunological Assessment of Vaccine Immunogenicity in the Draining Lymph Node.
    Article Snippet: Samples were acquired on a BD LSRFortessa using BD FACSDiva. .. For NHP B cell analysis, thawed LN single-cell suspensions were stained with LIVE/DEAD Aqua Viability Dye (Life Technologies) and the following surface Abs: IgD Alexa Fluor 488 (polyclonal, SouthernBiotech), CD20 allophycocyanin-Cy7 (2H7, BioLegend), CD14 BV510 (M5E2, BioLegend), CD3 Alexa Fluor 700 (SP34-2, BD), CD8a BV510 (RPA-T8, BioLegend), CD16 BV510 (3G8, BioLegend), CD10 BV510 (HI10a, BioLegend), IgG BV786 (G18-145, BD), CD95 BUV737 (DX2, BD), CD4 BV605 (L200, BD), CXCR5 PE-Cy7 (MU5UBEE, eBioscience), and PD-1 BV421 (EH12.2H7, BioLegend). .. S-specific B cells were identified using biotinylated SARS-CoV-2 S probe conjugated to streptavidin PE (Thermo Fisher Scientific).

    Single Cell:

    Article Title: Coformulation with Tattoo Ink for Immunological Assessment of Vaccine Immunogenicity in the Draining Lymph Node.
    Article Snippet: Samples were acquired on a BD LSRFortessa using BD FACSDiva. .. For NHP B cell analysis, thawed LN single-cell suspensions were stained with LIVE/DEAD Aqua Viability Dye (Life Technologies) and the following surface Abs: IgD Alexa Fluor 488 (polyclonal, SouthernBiotech), CD20 allophycocyanin-Cy7 (2H7, BioLegend), CD14 BV510 (M5E2, BioLegend), CD3 Alexa Fluor 700 (SP34-2, BD), CD8a BV510 (RPA-T8, BioLegend), CD16 BV510 (3G8, BioLegend), CD10 BV510 (HI10a, BioLegend), IgG BV786 (G18-145, BD), CD95 BUV737 (DX2, BD), CD4 BV605 (L200, BD), CXCR5 PE-Cy7 (MU5UBEE, eBioscience), and PD-1 BV421 (EH12.2H7, BioLegend). .. S-specific B cells were identified using biotinylated SARS-CoV-2 S probe conjugated to streptavidin PE (Thermo Fisher Scientific).

    Recombinase Polymerase Amplification:

    Article Title: Coformulation with Tattoo Ink for Immunological Assessment of Vaccine Immunogenicity in the Draining Lymph Node.
    Article Snippet: Samples were acquired on a BD LSRFortessa using BD FACSDiva. .. For NHP B cell analysis, thawed LN single-cell suspensions were stained with LIVE/DEAD Aqua Viability Dye (Life Technologies) and the following surface Abs: IgD Alexa Fluor 488 (polyclonal, SouthernBiotech), CD20 allophycocyanin-Cy7 (2H7, BioLegend), CD14 BV510 (M5E2, BioLegend), CD3 Alexa Fluor 700 (SP34-2, BD), CD8a BV510 (RPA-T8, BioLegend), CD16 BV510 (3G8, BioLegend), CD10 BV510 (HI10a, BioLegend), IgG BV786 (G18-145, BD), CD95 BUV737 (DX2, BD), CD4 BV605 (L200, BD), CXCR5 PE-Cy7 (MU5UBEE, eBioscience), and PD-1 BV421 (EH12.2H7, BioLegend). .. S-specific B cells were identified using biotinylated SARS-CoV-2 S probe conjugated to streptavidin PE (Thermo Fisher Scientific).



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